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Cell Signaling Technology (CST) scientists perform more than one thousand western blots daily to validate our existing and new antibodies. Our protocol that has been optimized for over a decade is available online so you can replicate our procedure and get reproducible and reliable results.Our Western Blotting Protocol shows you the critical experimental steps in western blotting and explains how small changes to the protocol can affect the final outcome of your blot.Additionally, you can downlo
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